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Cell culture experiments are widely used in biomedical
research, regenerative medicine, and biotechnological
production. Due to restrictions on the use of laboratory animals
by animal protection laws and the strict implementation of the
3Rs (Replacement, Reduction, and Refinement) formulated
by William Russell and Rex Burch to improve the welfare
of animals, it can be expected that the general use of cell
lines will further increase during the next years to substitute
animal-based research. However, it should be noted that cell
culture experiments, if not properly conducted, are prone to
errors. Therefore, it is essential that cell culture studies are
performed with good cell culture practice (GCCP) to assure
the reproducibility of in vitro experimentation.
In particular, inter- and intra-specific cross-contamination
and cell misidentification, genetic drift, contamination with
bacteria, fungi, yeast, viruses, or chemicals, and lack of quality
control testing are widespread fatal cell culture problems that
contaminate the literature with false and irreproducible results.
Rough estimates suggest that the number of published papers
that used problematic cell lines is about 16.1%. Moreover, the
International Cell Line Authentication Committee (ICLAC) lists
576 misidentified or cross-contaminated cell lines in its latest
register released in June 2021.
(Weiskirchen S, Schröder SK, Buhl EM, Weiskirchen R. A Beginner’s
Guide to Cell Culture: Practical Advice for Preventing Needless Problems.
Cells. 2023 Feb 21;12(5):682. doi: 10.3390/cells12050682.
PMID: 36899818; PMCID: PMC10000895.
www.ncbi.nlm.nih.gov/pmc/articles/PMC10000895/. Adaptado)